Regulated gene expression in the chicken embryo by using replication-competent retroviral vectors.
نویسندگان
چکیده
Rous sarcoma virus (RSV)-derived retroviral vector could efficiently deliver the green fluorescent protein (GFP), which is driven by the internal cytomegalovirus enhancer/promoter, into restricted cell populations in the chicken embryo. RSV-derived vectors coupled with the tet regulatory elements also revealed doxycycline-dependent inducible GFP expression in the chicken embryo in ovo.
منابع مشابه
Gene delivery into the chicken embryo by using replication-competent retroviral vectors.
Rous sarcoma virus (RSV)-derived retroviral vectors have allowed for efficient gene transfer into the chicken embryo which is a classical model for studying vertebrate development. Current evidence reveals that this method can be used for regionally restricted expression, inducible expression, and for interfering with endogenous gene function, suggesting that gain-of-function and loss-of-functi...
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ورودعنوان ژورنال:
- Journal of virology
دوره 76 4 شماره
صفحات -
تاریخ انتشار 2002